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first time DNA testing

Posted: Fri May 27, 2016 10:09 am
by slwatson
I recently sent off for some DNA testing for five birds, and it was not an easy experience. I chose to do the blood testing, where you have to clip one nail and send the blood to the company on these little cards. I had everything I needed on stand by, but the kwik stop powder wasn't stopping the bleeding as quickly as I would have liked, and I just felt horrible each time I clipped a nail!

I got them to stop bleeding as much as I could, but I was really worried I would find someone dead at the bottom of the cage the next day. When I got up I immediately checked the cages, and all five birds looked great! I turned to glance at my biggest flight, and my heart sank. My favorite male gouldian, whom I've had less than a month, dead at the bottom of the cage :(( :(( :(( Another unexplained loss.

Re: first time DNA testing

Posted: Fri May 27, 2016 10:40 am
by Babs _Owner
slwatson

Oh no Stacie, he was beautiful. =(( Did he seem thin? Get tangled in anything?

Re: first time DNA testing

Posted: Fri May 27, 2016 10:49 am
by debbie276
So sorry :cry:
He was beautiful

Re: first time DNA testing

Posted: Fri May 27, 2016 11:41 am
by Sally
So very sorry. I hate these unexplained losses. I think it is the not knowing that is almost the worst.

I also hate DNA testing. It is amazing how short you have to clip a nail to get some blood, but it actually only takes one drop, so I clip a little at a time till I get that little bit of blood.

Re: first time DNA testing

Posted: Fri May 27, 2016 1:54 pm
by slwatson
debbie276@Babs@Sally

He was perfectly fine when I went to bed that night. It was the craziest thing. I wonder if that cage got scared and he broke his neck or something.


@Sally

I tried so hard to not clip too far, but there were two that just bled and bled. Then they would try to fly away and splatter tiny droplets of blood :shock:

Re: first time DNA testing

Posted: Fri May 27, 2016 6:32 pm
by kyle
slwatson
He was a beauty. Sorry for your loss. What species were you DNA testing? Was it to determining the gender?

Re: first time DNA testing

Posted: Fri May 27, 2016 7:17 pm
by Jen
slwatson
Oh my goodness.....I HATE when the unexpected happens this way. At least if they are showing signs of illness, you can be prepared. I see he is a "blue"... I have not had one successful Blue live from my hatching this season....so heartbreaking. I have a lot of SPLITS....all very beautiful and healthy. Next season I am changing up the pairs so hopefully I will have some success. I am so sorry you lost your beautiful boy. :(

Re: first time DNA testing

Posted: Sat May 28, 2016 4:33 pm
by cjkrit
Hi, It is really not necessary to clip the nail.
Take a sharp thin disposable needle and deliver a quick prick just above the nail where the flesh starts- immediately a drop of blood will appear and that is all you need.
Be sure to use a new needle for every bird as not to get the results mixed up.

Re: first time DNA testing

Posted: Tue Jun 07, 2016 5:08 pm
by slwatson
kyle

I was dna testing parrot finches to determine gender. Sometimes the males will sing, but it's hard to catch them!

Re: first time DNA testing

Posted: Tue Jun 07, 2016 5:09 pm
by slwatson
cjkrit

Thank you so much for the suggestion! I'll try that next time :D

Re: first time DNA testing

Posted: Tue Jun 07, 2016 5:26 pm
by Sally
cjkrit wrote: Hi, It is really not necessary to clip the nail.
Take a sharp thin disposable needle and deliver a quick prick just above the nail where the flesh starts- immediately a drop of blood will appear and that is all you need.
Be sure to use a new needle for every bird as not to get the results mixed up.
I'd never heard of this, it would be so much easier than trying to cut a nail. I wonder if the tiny lancets that I use to test my blood sugar would work?

Re: first time DNA testing

Posted: Tue Jun 07, 2016 8:40 pm
by slwatson
Sally

I think it's worth a try...

Re: first time DNA testing

Posted: Wed Jun 08, 2016 12:26 am
by Sally
slwatson I'll bet they would, they are very fine, meant to bring up just a drop or so of blood on me, so I think they might do for the birds.

Re: first time DNA testing

Posted: Wed Jun 08, 2016 12:57 am
by cjkrit
Sally,
Those little lancets will be perfect for the job.
Remember a new one for every bird you test.

Re: first time DNA testing

Posted: Wed Jun 08, 2016 10:42 am
by Sally
cjkrit wrote: Sally,
Those little lancets will be perfect for the job.
Remember a new one for every bird you test.
These lancets actually will be easier. When I clip toenails, I have to disinfect the clippers between each bird, and many disinfectants are hard on metal. With the lancets, there will be no possibility of cross-contamination.